diff --git a/sentry_sdk/scope.py b/sentry_sdk/scope.py
index 0d3a0c6cd3..96b2bb5594 100644
--- a/sentry_sdk/scope.py
+++ b/sentry_sdk/scope.py
@@ -904,6 +904,10 @@ def span(self) -> "Optional[Span]":
     @span.setter
     def span(self, span: "Optional[Span]") -> None:
         self._span = span
+        if span is None:
+            self._transaction = None
+            self._transaction_info = {}
+            return
         # XXX: this differs from the implementation in JS, there Scope.setSpan
         # does not set Scope._transactionName.
         if isinstance(span, Transaction):
diff --git a/tests/tracing/test_misc.py b/tests/tracing/test_misc.py
index 9690f2ffc0..dd6bbc9f33 100644
--- a/tests/tracing/test_misc.py
+++ b/tests/tracing/test_misc.py
@@ -428,6 +428,9 @@ def test_start_transaction_updates_scope_name_source(sentry_init):
         assert scope._transaction == "foobar"
         assert scope._transaction_info == {"source": "route"}
 
+    assert scope._transaction is None
+    assert scope._transaction_info == {}
+
 
 @pytest.mark.parametrize("sampled", (True, None))
 def test_transaction_dropped_debug_not_started(sentry_init, sampled):