3DNuclei.po

# SOME DESCRIPTIVE TITLE.
# Copyright (C) 2023, Imaging Platform
# This file is distributed under the same license as the cellprofiler-tutorials package.
# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
# 
# Translators:
# Beth Cimini, 2024
# Marcelo Bispo de Jesus, 2026
# 
#, fuzzy
msgid ""
msgstr ""
"Project-Id-Version: cellprofiler-tutorials\n"
"Report-Msgid-Bugs-To: \n"
"POT-Creation-Date: 2026-02-19 20:20+0000\n"
"PO-Revision-Date: 2023-12-13 22:27+0000\n"
"Last-Translator: Marcelo Bispo de Jesus, 2026\n"
"Language-Team: Portuguese (Brazil) (https://app.transifex.com/center-for-open-bioimage-analysis/teams/169339/pt_BR/)\n"
"MIME-Version: 1.0\n"
"Content-Type: text/plain; charset=UTF-8\n"
"Content-Transfer-Encoding: 8bit\n"
"Language: pt_BR\n"
"Plural-Forms: nplurals=3; plural=(n == 0 || n == 1) ? 0 : n != 0 && n % 1000000 == 0 ? 1 : 2;\n"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:2
msgid "Analysis of noisy 3D nuclei"
msgstr "Análise de núcleos 3D em imagens com ruído"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:3
msgid ""
"**Tutorial for the Association of Biomolecular Research Facilities - Ligh "
"Microscopy Research Group (ABRF/LMRG)**"
msgstr ""
"**Tutorial para a Association of Biomolecular Research Facilities - Ligh "
"Microscopy Research Group (ABRF/LMRG)**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:5
msgid "**Proposal:**"
msgstr "**Proposta:**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:7
msgid "Segment the objects in a fluorescence z-stack image and provide:"
msgstr ""
"Segmentar os objetos em uma imagem de fluorescência em z-stack e fornecer:"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:9
msgid "Centroid location in microns (um) for each nucleus: x, y, and z c"
msgstr ""
"Localização do centroide em micrômetros (em micrômetros, µm) para cada "
"núcleo: x, y e z."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:10
msgid "Coordinates listed in separate columns. Labels x, y, and z."
msgstr "Coordenadas listadas em colunas separadas, rotuladas x, y e z."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:11
msgid "Integrated Intensity. Label intensity."
msgstr "Intensidade integrada, rotulada *intensity*."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:12
msgid "Volume in cubic microns (um^3). Label volume."
msgstr "Volume (em micrômetros cúbicos, µm^3), rotulado *volume*."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:13
msgid "3D volume image of your segmented image"
msgstr "Imagem volumétrica 3D do resultado da segmentação."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:15
msgid "**Input:**"
msgstr "**Entrada:**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:17
msgid ""
"Four different z-stack images that vary in their signal-to-noise ratio and "
"the clustering of their nuclei in 3D cell culture."
msgstr ""
"Quatro imagens z-stack diferentes que variam em sua relação sinal-ruído e no"
" agrupamento de seus núcleos em cultura de células 3D."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:20
msgid "Voxel dimensions: 0.124x0.124x0.200 um"
msgstr "Dimensões do voxel: 0,124x0,124x0,200 µm"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:22
msgid "**Importing data in CellProfiler**"
msgstr "**Importando dados no CellProfiler**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:24
msgid "Highlight the **Images** module."
msgstr "Selecione o módulo **Imagens**."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:26
msgid ""
"Drag-and-drop the images you will analyze into the **Images** module window."
msgstr ""
"Arraste e solte as imagens que você analisará na janela do módulo "
"**Imagens**."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:33
msgid ""
"Load the pipeline file (.cppipe). Drag-and-drop the file or go to "
"File->Import->Pipeline from File…"
msgstr ""
"Carregue o arquivo de pipeline (.cppipe). Arraste e solte o arquivo ou vá "
"para File->Import->Pipeline from File..."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:36
msgid ""
"Note: The pipeline should populate all information needed, but it’s always a"
" good practice to check if everything is fine."
msgstr ""
"Observação: o pipeline deve preencher todas as informações necessárias, mas "
"é sempre uma boa prática verificar se está tudo certo."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:39
msgid ""
"**Tip: Use the <img src=\"images/Info.png\" width=\"40\"/> button to learn "
"more or if you have questions.**"
msgstr ""
"**Dica: use o botão <img src=\"images/Info.png\" width=\"40\"/> para saber "
"mais ou se tiver dúvidas.**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:41
msgid "Highlight the **NamesAndTypes** module."
msgstr "Selecione o módulo **NamesAndTypes**."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:44
msgid ""
"The **NamesAndTypes** module gives each image a meaningful name by which "
"modules in the analysis pipeline will refer to it. This module will also let"
" you define an image stack that should be processed as a whole 3D volume."
msgstr ""
"No módulo **NamesAndTypes**, você dá nomes padronizados e representativos às"
" imagens, que serão usados como referência pelos demais módulos do pipeline."
" Esse módulo também permite definir uma pilha de imagens para ser processada"
" como um volume 3D completo."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:49
msgid ""
"Assign a name to: All images This is the simplest choice and the appropriate"
" one because we have only one kind of image."
msgstr ""
"Em 'Assign a name to:', selecione 'All images' para aplicar a nomeação a "
"todas as imagens.  Esta é a escolha mais simples e adequada porque temos "
"apenas um tipo de imagem."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:51
msgid "Process as 3D: Yes Selecting “Yes” will load the files as volumes"
msgstr ""
"Em 'Process as 3D:' selecione 'Yes', os arquivos serão carregados como "
"volumes"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:53
msgid ""
"Populate the fields for “Relative Pixel Spacing”. This calibration affects "
"modules that handle 3D images as volumes."
msgstr ""
"Preencha os campos de “Relative Pixel Spacing”. Essa calibração é importante"
" porque influencia os módulos que processam imagens 3D como um volume."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:56
msgid ""
"The relative pixel spacing was provided and is 0.124 um in x and y and 0.200"
" um in z."
msgstr ""
"O espaçamento relativo de pixels, Relative Pixel Spacing, fornecido é 0,124 "
"µm em x e y, e 0,200 µm em z. Para executar o arquivo de calibração, altere "
"temporariamente esse valor para 1 × 1 × 1 µm."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:59
msgid ""
"Assign the images “variable names” that describe the contents in the image. "
"For example, use the name \"Nuclei\", \"DNA\", “DAPI” or something else that"
" will remind you what the image is."
msgstr ""
"Atribua às imagens nomes descritivos que indiquem o conteúdo da imagem. Por "
"exemplo: \"Núcleos\", \"DNA\", “DAPI”, ou outro nome que ajude você a "
"reconhecer rapidamente o que aquela imagem representa."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:60
msgid "Hit the \"update\" button to populate"
msgstr "Clique em “Update” para preencher os campos automaticamente."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:68
msgid ""
"This analysis pipeline can be divided into three main parts: Image "
"processing,segmentation and measurement/export."
msgstr ""
"Este pipeline de análise pode ser dividido em três etapas principais: "
"processamento de imagens, segmentação e medição/exportação."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:76
msgid "**Image processing:**"
msgstr "**Processamento de imagens:**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:78
msgid ""
"The image set provided varies in signal-to-noise ratio, before attempting to"
" segment the nuclei in these images, conditioning the images with filters "
"and various image processing methods will improve segmentation results."
msgstr ""
"O conjunto de imagens fornecido apresenta variação na relação sinal-ruído. "
"Antes de tentar segmentar os núcleos nessas imagens, é recomendável aplicar "
"filtros e outras etapas de pré-processamento, pois isso melhora os "
"resultados da segmentação."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:83
msgid "Hit the <img src=\"images/StartTestMode.png\" width=\"150\"/> button."
msgstr "Clique no botão <img src=\"images/StartTestMode.png\" width=\"150\"/>."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:85
msgid ""
"**Tip:** You can select different image sets. For that you can hit the <img "
"src=\"images/NextImageSet.png\" width=\"150\"/> button, or you can go to the"
" main tab, hit the test tab and choose another image set  <br>"
msgstr ""
"**Dica:** você pode selecionar diferentes conjuntos de imagens. Para isso "
"você pode clicar no botão <img src=\"images/NextImageSet.png\" "
"width=\"150\"/> ou ir para a aba principal, clicar na aba de teste e "
"escolher outro conjunto de imagens \"Choose Image Set\"<br>"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:94
msgid ""
"**GaussianFilter** module This module will blur the image and remove part of"
" the noise. Filtering an image with a Gaussian filter can be helpful if the "
"foreground signal is noisy or near the noise floor."
msgstr ""
"**GaussianFilter** Este módulo aplica um desfoque gaussiano na imagem para "
"suavizar variações de intensidade e, com isso, reduzir parte do ruído. Ele é"
" especialmente útil quando o sinal do objeto de interesse (foreground) está "
"granulado/ruidoso ou muito próximo do nível de ruído (noise floor), o que "
"pode atrapalhar a segmentação."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:95
msgid "Select the input image: Nuclei (from **NamesAndTypes** module),"
msgstr ""
"Selecione a imagem de entrada (Select the input image): Nuclei (ou no nome "
"que você atribuiu no  módulo **NamesAndTypes**),"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:96
msgid "Name the output image: GaussianFilter,"
msgstr ""
"Nomear a imagem de saída em 'Name the output image:' como 'GaussianFilter',"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:97
msgid "Sigma: 1 (larger sigmas induce more blurring)."
msgstr "Sigma: 1 (sigmas maiores induzem mais desfoque)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:104
msgid ""
"Hit the <img src=\"images/Step.png\" width=\"150\"/> button and a new window"
" will pop up with a resulting image that should look like this. The image "
"can vary depending on the Image set chose."
msgstr ""
"Clique no botão <img src=\"images/Step.png\" width=\"150\"/> e uma nova "
"janela aparecerá com uma imagem resultante que deverá ser semelhante a esta."
" A imagem pode variar dependendo do conjunto de imagens escolhido."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:113
msgid ""
"The resulting image is still noisy in image set 3 and 4, so we added a "
"**ReduceNoise** module to perform a non-local mean noise reduction. Instead "
"of only using a neighborhood of pixels around a central pixel for denoising,"
" such as in **GaussianFilter**."
msgstr ""
"A imagem resultante ainda está com bastante ruído nos conjuntos de imagens 3"
" e 4, então adicionamos o módulo **ReduceNoise** para aplicar uma redução de"
" ruído do tipo non-local means. Diferente do **GaussianFilter**., que "
"suaviza a imagem considerando principalmente uma vizinhança local de pixels "
"ao redor de cada pixel."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:118
msgid ""
"**ReduceNoise** module. This module performs non-local means of noise "
"reduction. This will run a 5x5 pixel patch with a maximal distance of 2 "
"pixels to search for patches to use for denoising using a cut-off of 0.2."
msgstr ""
"**Reduzir Ruído**. Este módulo realiza redução de ruído por non-local means."
" Ele executará um patch de 5×5 pixels, com uma distância máxima de 2 pixels,"
" para procurar patches a serem usados na remoção de ruído, utilizando um "
"cut-off de 0,2."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:120
msgid ""
"**NOTE** This module is quite time consuming, don't worry if it takes "
"several seconds to run."
msgstr ""
"**NOTA** Este módulo consome bastante tempo, não se preocupe se demorar um "
"pouco para ser executado."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:122
msgid ""
"Select the output image: GaussianFilter (from **GaussianFilter** module)."
msgstr ""
"Em 'Select the output image:' selecione GaussianFilter (from "
"**GaussianFilter**)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:123
msgid "Name the output image: ReduceNoise"
msgstr "Em 'Name the output image:' escreva 'ReduceNoise'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:124
msgid "Size: 5"
msgstr "em 'Size:' escreva '5'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:125
msgid "Distance: 2"
msgstr "Em 'Distance:' escreva '2'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:126
msgid "Cut-off distance: 0.2"
msgstr "Em 'Cut-off distance:' escreva '0.2'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:133
msgid ""
"**Tip: The image tools on the top toolbar may be helpful to see the details "
"on your image/objects:**"
msgstr ""
"**Dica: as ferramentas de imagem na barra de ferramentas superior podem ser "
"úteis para ver os detalhes de sua imagem/objetos:**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:141
msgid ""
"The 1st icon from the left lets you reset the view back to the original "
"view."
msgstr ""
"O primeiro ícone à esquerda permite redefinir a visualização para a "
"visualização original."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:144
msgid ""
"The 2nd and 3rd icons let you step backwards and forwards through any "
"changes you made to the view."
msgstr ""
"O segundo e o terceiro ícones permitem que você volte e avance pelas "
"alterações que você fez na visualização."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:147
msgid ""
"The 4th icon lets you change the view by moving in any direction in the "
"display, by clicking and dragging."
msgstr ""
"O quarto ícone permite que você altere a visualização, movendo-a em qualquer"
" direção na tela ao clicar e arrastar."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:150
msgid ""
"The 5th icon lets you change the view by zooming, by dragging and drawing a "
"box to zoom in on."
msgstr ""
"O quinto ícone permite que você altere o zoom, arrastando e desenhando uma "
"caixa na área em que deseja ampliar."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:158
msgid "**Segmentation**"
msgstr "**Segmentação**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:160
msgid ""
"Now all objects on different image sets have similar dispersion patterns so "
"we can start the segmentation process. **Tip:** To view pixel intensities in"
" an open image, move your mouse over the image, the pixel intensities will "
"appear in the bottom bar of the display window."
msgstr ""
"Agora, todos os objetos nos diferentes conjuntos de imagens apresentam "
"padrões de dispersão semelhantes, então podemos iniciar o processo de "
"segmentação. **Dica:** Para visualizar as intensidades de pixel em uma "
"imagem aberta, mova o mouse sobre a imagem; as intensidades de pixel "
"aparecerão na barra inferior da janela de exibição."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:165
msgid ""
"**Threshold** module. This module produces a binary, or black and white, "
"image based on a threshold that can be pre-selected or calculated "
"automatically using one of many methods. After the threshold value has been "
"determined, the Threshold module will set pixel intensities below the value "
"to zero (black) and above the value to one (white)."
msgstr ""
"**Threshold**. Este módulo produz uma imagem binária, ou preto e branco, com"
" base em um limiar (threshold) que pode ser pré-selecionado ou calculado "
"automaticamente usando um dos vários métodos. Depois que o valor do limiar é"
" determinado, o módulo Threshold define as intensidades de pixel abaixo "
"desse valor como zero (preto) e acima desse valor como um (branco)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:166
msgid "Select the input image: ReduceNoise (from **ReduceNoise** module)"
msgstr ""
"em 'Select the input image:' selecione o método **ReduceNoise** (from "
"ReduceNoise)"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:167
msgid "Name the output image: Threshold"
msgstr "Em 'Name the output image:' selecione 'Threshold'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:168
msgid ""
"Threshold strategy: Global (We choose a global threshold strategy here "
"because our background is relatively uniform after the image processing "
"steps of this pipeline)."
msgstr ""
"Em 'Threshold strategy:' selecione 'Global' (escolhemos uma estratégia de "
"limite global aqui porque nosso background é relativamente uniforme após as "
"etapas de processamento de imagem deste pipeline)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:169
msgid ""
"Threshold method: Otsu (automatically-calculated threshold method because it"
" can adapt to changes in lighting conditions between images (in this case we"
" have images with different pixel intensities)."
msgstr ""
"Em 'Translated by MT:' selecione 'Otsu' (método de threshold calculado "
"automaticamente, que se adapta a variações de iluminação entre as imagens; "
"aqui, as imagens têm intensidades de pixel diferentes)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:170
msgid ""
"Two-class or three-class thresholding: Three classes (separate three major "
"pixel levels \\[foreground, mid-level and   background\\])."
msgstr ""
"Em 'Two-class or three-class thresholding:' selecione 'Three classes' "
"(separa pixels em três níveis: \\[primeiro plano, nível intermediário e "
"background\\])."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:172
msgid ""
"Assign pixels in the middle intensity: Background (the middle : intensity "
"pixels levels are noise in these images)."
msgstr ""
"Em ‘Assign pixels in the middle intensity:’ selecione ‘Background’ (os "
"pixels de intensidade intermediária são ruído nessas imagens)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:174
msgid ""
"Threshold smoothing scale: 1.5 (Smoothing improves the uniformity of the "
"resulting objects, by removing jagged edges caused by   noise in the "
"acquired image)."
msgstr ""
"Em ‘Threshold smoothing scale:’ escreva ‘1.5’ (a suavização melhora a "
"uniformidade dos objetos resultantes, removendo bordas irregulares causadas "
"por ruído na imagem adquirida)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:176
msgid "Threshold correction factor: 1"
msgstr "Em ‘Threshold correction factor:’ escreva ‘1’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:188
msgid ""
"**Watershed** module. This module is used to separate different objects in "
"an image, which in this case will assign which nuclei."
msgstr ""
"**Watershed**. Este módulo é usado para separar diferentes objetos em uma "
"imagem; neste caso, para separar quais pixels pertencem a cada núcleo."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:189
msgid "Use advanced settings: No"
msgstr "Em ‘Use advanced settings:’ selecione ‘No’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:190
msgid "Select the input image: Threshold (from **Threshold** module)"
msgstr ""
"Em ‘Select the input image:’ selecione ‘Threshold’ (do módulo "
"**Threshold**)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:191
msgid "Name the output object: Watershed"
msgstr "Em ‘Name the output object:’ escreva ‘Watershed’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:192
msgid ""
"Generate from: Distance (we don’t have markers to guide the segmentation "
"process so the markers and other inputs for the algorithm will be "
"automatically generated based on the footprint size)."
msgstr ""
"Em ‘Generate from:’ selecione ‘Distance’ (como não temos marcadores para "
"guiar a segmentação, os marcadores e outras entradas do algoritmo serão "
"gerados automaticamente com base no tamanho do footprint)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:193
msgid ""
"Footprint: 10 (define the dimensions of the window used to scan the input "
"image for local maxima, this will create a local maxima from a binary image "
"that will be at the centers of objects. Large footprint will suppress local "
"maximas that are close together into a single maxima, so two or more objects"
" will be segmented as one. Small footprint can lead to oversegmenation, this"
" means one nuclei segmented as two or more objects."
msgstr ""
"Em ‘Footprint:’ escreva ‘10’ (define as dimensões da janela usada para "
"varrer a imagem de entrada em busca de máximos locais; isso cria máximos "
"locais a partir da imagem binária, que ficam no centro dos objetos. Um "
"footprint grande suprime máximos locais muito próximos, juntando-os em um "
"único máximo; assim, dois ou mais objetos podem ser segmentados como um só. "
"Um footprint pequeno pode levar à oversegmentation, ou seja, um núcleo ser "
"segmentado como dois ou mais objetos)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:195
msgid "Downsample: 3 (reduce processing time without losing data)."
msgstr ""
"Em ‘Downsample:’ escreva ‘3’ (reduz o tempo de processamento sem perder "
"dados)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:207
msgid ""
"**Note:** Sometimes the processing and the segmentation steps can shrink or "
"dilate your original structures. In this pipeline the objects are shrinked "
"after the segmentation, you could test this using an image with a known "
"object size."
msgstr ""
"**Nota:** Às vezes, as etapas de processamento e segmentação podem encolher "
"ou dilatar as estruturas originais. Neste pipeline, os objetos ficam menores"
" após a segmentação; você pode testar isso usando uma imagem com um objeto "
"de tamanho conhecido."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:212
msgid "**DilateObjects** module. This module dilates your objects and"
msgstr "**DilateObjects**. Esse módulo dilata os objetos e"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:214
msgid ": smooths the edges."
msgstr ": suaviza as bordas."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:215
msgid "Select the input object: Watershed (from **Watershed** module)"
msgstr ""
"Em ‘Select the input object:’ selecione ‘Watershed’ (do módulo "
"**Watershed**)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:216
msgid "Name the output object: RealsizeNuclei"
msgstr "Em 'Nomeie o objeto de saída:'selecione 'RealsizeNuclei'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:217
msgid ""
"Structuring element: Octahedron (Size: 1) (dilate the objects using an "
"octahedron profile with a size of 1)."
msgstr ""
"Em ‘Structuring element:’ selecione ‘Octahedron’ e em ‘Size:’ selecione ‘1’ "
"(dilata os objetos usando um elemento estruturante em forma de octaedro com "
"tamanho 1)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:229
msgid ""
"**Note**: The colors are assigned at random and will vary in each run of the"
" module."
msgstr ""
"**Nota**: As cores são atribuídas aleatoriamente e variam em cada execução "
"do módulo."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:231
msgid "**Measure and export data**"
msgstr "**Medindo e exportando os dados**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:233
msgid ""
"Now that the nuclei have been segmented, measurements can be made using "
"modules from the **Measurements** category. This study is asking for 3 "
"particular measurements:"
msgstr ""
"Agora que os núcleos foram segmentados, as medidas podem ser feitas usando "
"módulos **Measurements**. Este estudo fará 3 medidas específicas:"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:237
msgid "Centroid location (x, y and z)"
msgstr "Localização centróide (x, y e z)"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:239
msgid "Integrated intensity"
msgstr "Intensidade integrada"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:241
#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:384
msgid "Volume"
msgstr "Volume"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:243
msgid ""
"In this case we need two modules to extract this information, "
"**MeasureObjectIntensity** and **MeasureObjectSizeShape** modules."
msgstr ""
"Nesse caso, precisamos de dois módulos para extrair essas informações, os "
"módulos **MeasureObjectIntensity** e **MeasureObjectSizeShape**."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:246
msgid ""
"**Note:** When applying these measurements, be careful to measure the "
"original images, not rescaled or processed images."
msgstr ""
"**Nota:** Ao aplicar essas medidas, tome cuidado para medir as imagens "
"originais, não as imagens redimensionadas ou processadas."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:249
msgid "**MeasureObjectIntensity** module"
msgstr "Módulo **MeasureObjectIntensity**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:251
msgid ""
"Select images to measure: Nuclei (original image from **NamesAndTypes** "
"module)"
msgstr ""
"Em ‘Select images to measure:’ selecione ‘Nuclei’ (imagem original do módulo"
" **NamesAndTypes**)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:252
msgid ""
"Select objects to measure: RealsizeNuclei (from **DilateObjects** module) to"
" have all the intensity measurements from the object."
msgstr ""
"Abaixo, em ‘Select objects to measure:’ selecione ‘RealsizeNuclei’ (do "
"módulo **DilateObjects**), para obter todas as medidas de intensidade do "
"objeto."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:259
msgid ""
"**Note:** The measure modules will provide several features for identified "
"objects and at this point we cannot choose which measurement, so the module "
"will extract all intensity features possible."
msgstr ""
"**Nota:** Os módulos de medida fornecem várias features para os objetos "
"identificados e, neste ponto, não é possível escolher quais medidas extrair;"
" portanto, o módulo irá extrair todas as features de intensidade possíveis."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:268
msgid "**MeasureObjectSizeShape module**"
msgstr "Módulo **MeasureObjectSizeShape**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:270
msgid ""
"Select object sets to measure: RealsizeNuclei (from **DilateObjects** "
"module)"
msgstr ""
"Em 'Select object sets to measure:' selecione 'RealsizeNuclei' (do módulo "
"**DilateObjects**)"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:271
msgid "Calculate the Zernike features: No"
msgstr "Em 'Calculate the Zernike features:'selecione 'No'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:272
msgid "Calculate the advanced features: No"
msgstr "Em 'Calculate the advanced features:' selecione 'No'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:284
msgid "**Creating visuals**"
msgstr "**Criando de recursos visuais**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:286
msgid ""
"Add the **RescaleIntesity** module to your pipeline. This module lets you "
"rescale the intensity of the input images by any of several methods. You "
"should use caution when interpreting intensity and texture measurements "
"derived from images that have been rescaled because certain options for this"
" module do not preserve the relative intensities from image to image."
msgstr ""
"Adicione o módulo **RescaleIntesity** ao seu pipeline. Este módulo permite "
"redimensionar a intensidade das imagens de entrada usando vários métodos. "
"Você deve ter cuidado ao interpretar medidas de intensidade e textura "
"derivadas de imagens que foram redimensionadas porque certas opções deste "
"módulo não preservam as intensidades relativas de imagem para imagem."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:287
msgid "Select the input image image: Nuclei (from **NamesAndTypes** module)"
msgstr ""
"Em 'Select the input image image:' selecione 'Nuclei' (do módulo "
"**NamesAndTypes**)"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:288
msgid "Name the output image: RescaleIntensityNuclei"
msgstr "Em 'Name the output image:'escolha 'RescaleIntensityNuclei''"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:289
msgid ""
"Rescaling method: Stretch each image to use the full intensity range (Find "
"the minimum and maximum values within the unmasked part of the image (or the"
" whole image if there is no mask) and rescale every pixel so that the "
"minimum has an intensity of zero and the maximum has an intensity of one)."
msgstr ""
"Em ‘Rescaling method:’ selecione ‘Stretch each image to use the full "
"intensity range’ (esse método encontra os valores mínimo e máximo na parte "
"sem máscara da imagem, ou na imagem inteira, se não houver máscara, e "
"reescala os pixels para que o mínimo tenha intensidade 0 e o máximo tenha "
"intensidade 1)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:301
msgid ""
"**OverlayObjects** module. This module overlay the objects as colored masks "
"on the image. We recommend overlaying onto rescaled images,which will be "
"easier to visualize outside of CellProfiler."
msgstr ""
"**OverlayObjects**. Este módulo sobrepõe os objetos como máscaras coloridas "
"sobre a imagem. Recomendamos sobrepor as imagens reescaladas, pois elas "
"ficam mais fáceis de visualizar fora do CellProfiler."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:302
msgid "Input: RescaleIntensityNuclei (from **RescaleIntensity** module)"
msgstr ""
"Em 'Input:' selecione 'RescalelntensityNuclei' (do módulo "
"**RescaleIntensity**)"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:303
msgid "Name the output image: OverlayObjects"
msgstr "Em 'Name the output image:' escreva 'OverlayObjects'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:304
msgid "Objects: RealsizeNuclei (from **DilateObjects** module)"
msgstr ""
"Em 'Objects:' selecione 'RealsizeNuclei' (do módulo **DilateObjects**)"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:305
msgid ""
"Opacity: 0.3 (Increase this value to decrease the transparency of the "
"colorized object labels)."
msgstr ""
"Em 'Opacity:' escreva '0,3' (aumente este valor para diminuir a "
"transparência dos rótulos dos objetos coloridos)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:312
msgid ""
"**SaveImages** module. This module saves image or movie files. Because "
"CellProfiler usually performs many image analysis steps on many groups of "
"images, it does not save any of the resulting images to the hard drive "
"unless you specifically choose to do so with the SaveImages module. You can "
"save any of the processed images created by CellProfiler during the analysis"
" using this module. You can choose from many different image formats for "
"saving your files. This allows you to use the module as a file format "
"converter, by loading files in their original format and then saving them in"
" an alternate format."
msgstr ""
"Módulo **SalvarImagens**. Este módulo salva arquivos de imagem ou vídeo. "
"Como o CellProfiler normalmente executa muitas etapas de análise em muitos "
"conjuntos de imagens, ele não salva automaticamente no disco rígido nenhuma "
"das imagens resultantes, a menos que você escolha fazer isso com o módulo "
"‘SaveImages’. Com este módulo, você pode salvar qualquer imagem processada "
"criada pelo CellProfiler durante a análise. Também é possível escolher entre"
" vários formatos de arquivo, o que permite usar o módulo como um conversor: "
"você carrega os arquivos no formato original e depois os salva em um formato"
" desejado."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:314
msgid "Select the type of image to save: Image"
msgstr "Em ‘Select the type of image to save:’ selecione ‘Image’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:315
msgid ""
"Select the image to save: OverlayObjects (from **OverlayObjects** module)"
msgstr ""
"Em ‘Select the image to save:’ selecione ‘OverlayObjects’ (do módulo "
"**OverlayObjects**)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:316
msgid ""
"Select method for constructing file names: From image filename (use this "
"option to avoid reassignment of your images)"
msgstr ""
"Em ‘Select method for constructing file names:’ selecione ‘From image "
"filename’ (use esta opção para evitar a reatribuição das suas imagens)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:317
msgid ""
"Select image name for file prefix: Nuclei (select the original image name "
"from NamesAndTypes module)"
msgstr ""
"Em ‘Select image name for file prefix:’ selecione ‘Nuclei’ (selecione o nome"
" da imagem original no módulo ‘NamesAndTypes’)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:318
msgid "Append a suffix to the image file name?: Yes"
msgstr "Em ‘Append a suffix to the image file name?:’ selecione ‘Yes’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:319
msgid ""
"Text to append to the image name: Overlay (just add Overlay in the end of "
"your original image file name)"
msgstr ""
"Em ‘Text to append to the image name:’ escreva ‘Overlay’ (isso adiciona "
"‘Overlay’ ao final do nome original do arquivo de imagem)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:320
msgid ""
"Saved file format: tiff (tiff is a lossless format, but you can choose "
"others depending on what you need to do with this images)"
msgstr ""
"Em ‘Saved file format:’ selecione ‘tiff’ (tiff é um formato lossless, mas "
"você pode escolher outros dependendo do que precisa fazer com essas "
"imagens)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:321
msgid ""
"Image bit depth: 8-bit integer (this bit depth is easily read outside Cell "
"Profiler)"
msgstr ""
"Em ‘Image bit depth:’ selecione ‘8-bit integer’ (essa profundidade de bits é"
" facilmente lida fora do CellProfiler)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:322
msgid "Save with lossless compression: Yes"
msgstr "Em ‘Save with lossless compression:’ selecione ‘Yes’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:323
msgid ""
"Output file location: Default Output Folder (or create a new folder just for"
" this images)"
msgstr ""
"Em ‘Output file location:’ selecione ‘Default Output Folder’ (ou crie uma "
"nova pasta apenas para essas imagens)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:324
msgid ""
"Overwrite existing files without warning?: No (prevent file overwritten)"
msgstr ""
"Em ‘Overwrite existing files without warning?:’ selecione ‘No’ (evita "
"sobrescrever arquivos existentes)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:325
msgid "When to save: Every cycle (Save every image set)"
msgstr ""
"Em ‘When to save:’ selecione ‘Every cycle’ (salva a cada conjunto de "
"imagens)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:326
msgid "Record the file and path information to the saved image?: No"
msgstr ""
"Em ‘Record the file and path information to the saved image?:’ selecione "
"‘No’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:327
msgid "Create subfolders in the output folder: No"
msgstr "Em 'Create subfolders in the output folder:' selecione 'No'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:334
msgid ""
"**ConvertObjectsToImage** module. Transform objects in image (provide a 3D "
"volume image of the segmented image)"
msgstr ""
"**ConvertObjectsToImage**. Este módulo transforma objetos em uma imagem "
"(gera uma imagem de volume 3D a partir do objeto segmentado)."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:336
msgid ""
"Select the input objects: RealsizeNuclei (from **DilateObjects** module)"
msgstr ""
"Em ‘Select the input objects:’ selecione ‘RealsizeNuclei’ (do módulo "
"**DilateObjects**)"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:337
msgid "Name the output image: NucleiObjects3D"
msgstr "Em ‘Name the output image:’ escreva ‘NucleiObjects3D’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:338
msgid "Select the color format: Grayscale"
msgstr "Em ‘Select the color format:’ selecione ‘Grayscale’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:345
msgid "**Export measurements**"
msgstr "**Exportando as medidas**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:347
msgid ""
"It’s good practice to place all export modules at the end of your pipeline. "
"CellProfiler automatically calculates execution times for each module that "
"was run before the export module. By placing your export modules at the end "
"of your pipeline, you will have access to module execution times for each "
"module in your pipeline. Save the output of the measurement modules using "
"**ExportToSpreadsheet** or **ExportToDatabase**."
msgstr ""
"É uma boa prática colocar todos os módulos de exportação no final de seu "
"pipeline. O CellProfiler calcula automaticamente os tempos de execução de "
"cada módulo que foi executado antes do módulo de exportação. Ao colocar os "
"módulos de exportação no final do pipeline, você terá acesso aos tempos de "
"execução de cada módulo do pipeline. Salve a saída dos módulos de medição "
"usando **ExportToSpreadsheet** ou **ExportToDatabase**."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:355
msgid ""
"**ExportToSpreadsheet** module. This module exports measurements into one or"
" more files that can be opened in Excel or other spreadsheet programs. This "
"module will convert the measurements to a comma-, tab-, or other character-"
"delimited text format and save them to the hard drive in one or several "
"files, as requested."
msgstr ""
"**ExportToSpreadsheet**. Este módulo exporta as medidas para um ou mais "
"arquivos que podem ser abertos no Excel ou em outros programas de planilha. "
"Ele converte as medidas para um formato de texto delimitado por vírgula, "
"tabulação ou outro caractere e salva no disco rígido em um ou vários "
"arquivos, conforme configurado."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:361
msgid "Select the column delimiter: Comma (“,”)"
msgstr "Em ‘Select the column delimiter:’ selecione ‘Comma (\",\")'."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:363
msgid "Output file location: Default Output Folder"
msgstr "Em ‘Output file location:’ selecione ‘Default Output Folder’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:365
msgid "Add a prefix to file names: Yes"
msgstr "Em ‘Add a prefix to file names:’ selecione ‘Yes’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:367
msgid "Filename prefix: MyExpt\\_"
msgstr "Em ‘Filename prefix:’ escreva ‘MyExpt\\_'."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:369
msgid "Overwrite existing files without warning: No"
msgstr "Em ‘Overwrite existing files without warning:’ selecione ‘No’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:371
msgid "Add image metadata columns to your object data file: No"
msgstr ""
"Em ‘Add image metadata columns to your object data file:’ selecione ‘No’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:373
msgid "Add image file and folder names to your object data file: No"
msgstr ""
"Em ‘Add image file and folder names to your object data file:’ selecione "
"‘No’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:375
msgid "Representation of Nan/Inf: NaN"
msgstr "Em ‘Representation of Nan/Inf:’ selecione ‘NaN’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:377
msgid "Select the measurements to export: Yes"
msgstr "Em ‘Select the measurements to export:’ selecione ‘Yes’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:379
msgid "Press the button to select measurements:"
msgstr ""
"Clique no botão ‘Press the button to select measurements:’  para selecionar "
"as medidas."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:381
msgid "RealsizeNuclei:"
msgstr "Clique em 'RealsizeNuclei:'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:383
msgid "AreaShape: Center: X, Y and Z"
msgstr ""
"Clique em 'AreaShape:' depois em 'Center:' e selecione as opções: 'X, Y e Z'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:386
msgid "Intensity: IntegratedIntensity"
msgstr "Clique em 'Intensidade:' e selecione 'IntegratedIntensity'"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:388
msgid "Calculate the per-image mean values for object measurements?:No"
msgstr ""
"Em ‘Calculate the per-image mean values for object measurements?:’ selecione"
" ‘No’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:390
msgid "Calculate the per-image median values for object measurements?:No"
msgstr ""
"Em ‘Calculate the per-image median values for object measurements?:’ "
"selecione ‘No’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:392
msgid ""
"Calculate the per-image standard deviation values for object "
"measurements?:No"
msgstr ""
"Em ‘Calculate the per-image standard deviation values for object "
"measurements?:’ selecione ‘No’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:394
msgid "Output file location:Default Output Folder|"
msgstr "Em ‘Output file location:’ selecione ‘Default Output Folder’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:396
msgid "Create a GenePattern GCT file?:No"
msgstr "Em ‘Create a GenePattern GCT file?:’ selecione ‘No’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:398
msgid "Export all measurement types?:No"
msgstr "Em ‘Export all measurement types?:’ selecione ‘No’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:400
msgid "Data to export: RealsizeNuclei"
msgstr "Em ‘Data to export:’ selecione ‘RealsizeNuclei’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:402
#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:408
msgid "Use the object name for the file name?: No"
msgstr "Em ‘Use the object name for the file name?:’ selecione ‘No’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:404
msgid "File name: Nuclei.csv"
msgstr "Em ‘File name:’ escreva ‘Nuclei.csv’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:406
msgid "Data to export: Experiment"
msgstr ""
"Clique em ‘Add another data set’, em ‘Use the object name for the file "
"name?’ selecione ‘No’ e em ‘Data to export:’ selecione ‘Experiment’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:410
msgid "File name: Metadata.csv"
msgstr "Em ‘File name:’ escreva ‘Metadata.csv’."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:417
msgid "**Results overview:**"
msgstr "**Visão geral dos resultados:**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:424
msgid "**Congratulations!**"
msgstr "**Parabéns!**"

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:425
msgid "The nucleus has been segmented, measured and exported."
msgstr "O núcleo foi segmentado, as medidas foram realizadas e exportadas."

#: ../../source/3DNuclei/Nuclei3DTimeConsumingPipeline.md:428
msgid "**The End**"
msgstr "**Terminamos**"